Research Technologist
Johns Hopkins University
The Division of Pulmonary is seeking a Research Technologist to provide operational support in the Sidhaye Lab. The technologist will oversee the maintenance of the mouse colonies. In addition, will utilize a foundation in basic laboratory techniques to work with molecular biology and primary cell culture requiring a high level of rigor and reproducibility. Assist with the planning of experiments and interpreting results. Perform data collection, prepare reports and interface with sponsors for laboratory projects.
Specific Duties & Responsibilities
- Perform mouse breeding and husbandry.
- Perform general molecular biology techniques such as cloning, qPCR, and Western blotting.
- Conduct experiments involving propagation of tissue culture, plasmid constructions, gel electrophoresis, immunoblotting, immunohistochemical staining of cells and tissue, and RT-qPCR. (Research involves animal and human tissue).
- Follow established procedures or protocols and utilizes aseptic technique to perform laboratory tests and/or experiments which may include basic tissue culture, basic molecular biology, and specialized histology techniques.
- Operate basic laboratory equipment such as centrifuge, pH meter, analytical balance, incubator, spectrophotometer, light microscope, and/or utilizes laminar flow hood.
- Operate lab computers for data entry.
- Utilize in vivo models in experiments. Following protocols, monitor animals and maintain health records in accordance with USDA and AALAC guidelines.
- Sacrifice and dissect animal remains as required.
- Prepare tissue for study utilizing biochemical and histochemical techniques.
- Maintain accurate data records.
- Maintain and make minor adjustments to equipment, e.g., calibrating, alignment.
- Comply with biohazard/radiation safety standards through proper handling of potentially hazardous chemical and biological agents and/or radiation sources in the workplace.
- Complete annual university biohazard/universal precaution/radiation safety training, as appropriate.
- Use sterile techniques to avoid contaminating lab experiments.
- Use universal safety precautions to protect self and co-workers from biohazardous materials, including blood-borne pathogens.
- Prepare solutions, reagents, and stains following standard laboratory formulas and procedures.
- Monitor inventory levels, order materials and supplies, count orders upon receipt.
- Perform multiple tasks efficiently and correctly, often operating more than one instrument at a time.
- Provide training to other individuals who may be assigned to the laboratory on a regular or temporary basis.
Minimum Qualifications
- Bachelor's Degree in biology, chemistry or related field
- Experience in basic laboratory procedures.
- Additional education may substitute for required experience and additional related experience may substitute for required education beyond HS Diploma/Graduation Equivalent, to the extent permitted by the JHU equivalency formula.
Preferred Qualifications
- Experience working with in vivo models
- Some related post-undergraduate work experience.
- Experience in a comparable laboratory, or a significant independent research project, not related to normal course requirements.
Classified Title: Research Technologist
Role/Level/Range: ACRO40/E/02/CC
Starting Salary Range: $15.70 - $26.25 HRLY (Commensurate w/exp.)
Employee group: Full Time
Schedule: Monday to Friday 8:30am to 5pm. Weekends may be required.
FLSA Status: Non-Exempt
Location: School of Medicine Campus
Department name: SOM DOM Pulmonary
Personnel area: School of Medicine