Lab Research Tech II
University of Pittsburgh
The University of Pittsburgh, Department of Immunology, is seeking a full-time technician to support newly funded research programs. The Cortez lab investigates mucosal tissue immune responses in homeostasis and during infection, focusing primarily on innate lymphoid cell responses. There are opportunities for independent projects within the mission of the laboratory, based on the applicant’s skills and goals, and after a period of guided supervision. Applicants that are interested in having their own independent project are encouraged to apply.
The applicant will support the function of the laboratory and assist scientists conducting experiments in cellular and molecular immunology. The applicant should have some experience with flow cytometry and basic immunological techniques. A significant task will include maintenance of the mouse colony, which includes breeding, PCR-based genotyping, and record keeping. The applicant should be very eager to learn new techniques and improve existing techniques.
Other required skills: Excellent organizational and planning skills, and attention to detail, are essential. Strong mathematical skills related to the biological sciences, including dilutions, ratios, concentrations, etc. Must keep detailed and accurate records. Excellent writing and verbal communication. Proven ability to work in a collaborative team, with some independent tasks. Experience with University of Pittsburgh systems and processes for purchasing and ordering is preferred.
Job Summary
Performs a variety of laboratory experiments and procedures. Prepares specimens. Oversees supply management. Operates and properly maintains equipment.
1. Perform supervised laboratory work and experiments, including guided analysis and interpretation of murine and human cell culture experiments. These will involve cell isolation from organs, immunofluorescence staining, flow cytometry, cell sorting, cell culture, and other techniques. Writes reports and summaries of findings with guidance from supervisor.
2. Oversees specimen inventories.
3. Maintains lab and chemical inventories. Assists with ordering as needed.
4. Work with supervisor to prepare data for presentation to the group and for publication.
5. Assist other lab members in basic immunological procedures.
Minimal Physical Effort