Research Technician I
University of Rochester
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Job Location (Full Address):
601 Elmwood Ave, Room G9872, Rochester, New York, United States of America, 14642Opening:
Worker Subtype:
TemporaryTime Type:
Full timeScheduled Weekly Hours:
40Department:
400820 Dept of Biomedical GeneticsWork Shift:
UR - Day (United States of America)Range:
UR URG 104 HCompensation Range:
$18.65 - $26.11The referenced pay range represents the minimum and maximum compensation for this job. Individual annual salaries/hourly rates will be set within the job's compensation range, and will be determined by considering factors including, but not limited to, market data, education, experience, qualifications, expertise of the individual, and internal equity considerations.
Responsibilities:
GENERAL PURPOSEThe individual will be part of a research team working to understand the role of antioxidants in colitis using cutting edge technologies. Duties will include laboratory work related in the field of inflammatory diseases, with a focus on ulcerative colitis (UC) and Crohn’s disease (CD). Among other diseases, our lab studies the role of antioxidants in colitis. Our approach uses both animal models of UC/CD, as well as in vitro models of intestinal organoids. The individual will perform a variety of moderately complex laboratory experiments. With an excellent track record in placing prior team members, our lab and this position is uniquely suited as a launching point for research (PhD) or medicine (MD), and enables entry into a future career in regenerative medicine (both academia and industry).
Procedures performed and equipment used:
UC/CD model mouse colony maintenance, genotyping, histology. Tissue dissection, cell isolation and purification, cell culture procedures and genetic manipulation of cells. RNA isolation and RT-QPCR, protein isolation, gel electrophoresis, image capture and analysis (CX5), immunofluorescent analyses. No involvement in the assessment, treatment or care of patients is involved with this work.
Supervision:
This position reports to Dr. Isaac Harris, Ph.D. After a period of initial guidance and training, this position requires that the individual be able to execute experiments independently, analyze data, and help troubleshoot results. This person will also occasionally help train and/or guide others in existing lab techniques.
ESSENTIAL FUNCTIONS
- Helps to maintain mouse animal colonies: routine animal mating and husbandry logs, genotyping of offspring, tissue harvest and histology.
- Plans and performs a variety of cellular and molecular experiments and analyses investigating human disease biology including but not limited to: analysis of cell division and survival, gene expression analysis, genetic modification of cells using transient transfection and lentiviral vectors, drug
- screening and in vitro modeling of neural cell functions.
- Performs routine cell culture preparations of intestinal organoid tissues.
- Maintains detailed lab journal. Records and calculates results. Presents data in prescribed formats.
- Extracting from data, literature searches and existing resources to come up with reasoning to interpret experimental observations or problems that may be encountered. Uses theoretical knowledge to trouble shoot experiments and
- equipment malfunctions. Independence in problem solving is highly encouraged.
- Performs general laboratory duties such as preparing reagents and re-stocking supplies and maintenance of cryopreservation systems. Helps to ensure laboratory compliance for biosafety, EHS and fire inspections.
- Provides assistance and training for incoming students.
- Participates in lab and research meetings.
Other duties as assigned
QUALIFICATIONS
- High school diploma or GED required.
- BA/BS in Biochemistry, Chemistry, Biology, Neuroscience, Biomedical Engineering, or a related field preferred.
- Or equivalent combination of education and experience required.
- One-year experience performing basic sampling, monitoring, or research activities, compiling and collecting data, and preparing reports preferred.
- Highly motivated, ability to multitask, good organizational abilities, and ability to work well as part of a team preferred.
- Strong interpersonal and communication skills, including written communication preferred.
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