Research Assistant, Biochemistry, Karbstein
Vanderbilt University
About the Work Unit:
The Karbstein Lab in the Department of Biochemistry at Vanderbilt University is seeking an enthusiastic molecular biology/biochemistry research assistant to aid in the lab's studies of ribosome assembly, quality control and repair. In addition to providing a better understanding about the cellular mechanisms underlying ribosome integrity, this basic science work is highly relevant to cancer biology, the biology of aging. The lab relies heavily on biochemistry and molecular biology, as well as yeast genetics. For examples of the type of research that will be undertaken see: Blomqvist et al, 2023, PLoS Genetics; Yang et al, 2023, Mol Cell; and Parker et al, 2024, PLoS Biology; Yang&Karbstein, BioRxiv 2024.
The lab is a mid-sized lab seeking a research associate who will spend their time performing research, with some time spent maintaining lab infrastructure. The new research associate will be directly mentored and instructed by Dr. Karbstein, the Principle Investigator, and plan, conduct, interpret and present research. The laboratory is a supportive, fast-paced environment for driven candidates who enjoy a collaborative atmosphere. It is expected that the new research associate will produce enough data for a first author publication within 1-2 years of joining the lab.
The lab strives to be a diverse, inclusive, and equitable working environment; prioritizes personal growth and wellness; and is committed to work-life balance. Overall, this is an excellent opportunity for an experienced researcher who enjoys the independence of an academic lab.
Key Functions and Expected Performances:
Research and Development:
- Purifies ribosomes from various organisms for mass spectrometry analysis
- Experience in RNA biochemistry is an asset
- Uses protein gels, Western blotting and mass spectrometry to analyze ribosome assembly factors and ribosomal proteins
- Uses Northern blotting to analyze ribosome assembly in cells
- Uses agarose gels to analyze PCR screens and PCR reactions
- Generates yeast and worm strains and human cell lines
- Uses quantitative growth measurements to monitor genetic interactions
- Designs and clones proteins and RNAs for yeast, worm or human in vivo work and in vitro assays
- Analyzes RNAseq data
- Analyzes experiments independently and prepares them for presentation to the PI
Supervisory Relationships:
Might supervise interns, work-study students or undergraduate researchers.
The position reports administratively and functionally to the PI
Education and Certifications:
- A Bachelor’s degree in cellular biology, molecular biology, biochemistry, or a related field from an accredited institution of higher education and a minimum of four years of experience is required.
Experience and Skills:
- Ability to perform all basic calculations related to buffer composition (Molarity, dilution factor, etc.) is necessary.
- Experience with protein and/or RNA biochemistry is strongly preferred.
- Computational experience is preferred.
- Being comfortable in learning new assays is necessary.
At Vanderbilt University , our work - regardless of title or role - is in service to an important and noble mission in which every member of our community serves in advancing knowledge and transforming lives on a daily basis. Located in Nashville, Tennessee, on a 330+ acre campus and arboretum dating back to 1873, Vanderbilt is proud to have been named as one of “America’s Best Large Employers” as well as a top employer in Tennessee and the Nashville metropolitan area by Forbes for several years running. We welcome those who are interested in learning and growing professionally with an employer that strives to create, foster and sustain opportunities as an employer of choice.
We understand you have a choice when choosing where to work and pursue a career. We understand you are unique and have a story. We want to hear it. We encourage you to apply today so that you might become a part of our story.
The Karbstein lab in the Department of Biochemistry at Vanderbilt University is seeking a talented biochemistry Research Assistant to join our group. This individual will work closely with our team of students and postdocs to investigate mechanisms of ribosome assembly and repair. This position is well-suited applicants with an interest in basic cellular mechanisms, cancer biology, or cellular aging, and we are open to hiring at all levels.